Powdered Decaffeinated Green Tea Extract

Author: Marina

Apr. 29, 2024

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Powdered Decaffeinated Green Tea Extract

Packaging and storage—

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Preserve in well-closed containers, protected from light and moisture, and store at controlled room temperature.

The label states the Latin binomial and, following the official name, the part of the plant contained in the article. It meets other labeling requirements under Botanical Extracts 565

The retention times of the peaks for ()-epigallocatechin, (+)-catechin, ()-epicatechin, ()-epigallocatechin-3--gallate, ()-gallocatechin-3--gallate, ()-epigallocatechin-3--(3-methyl)-gallate, and ()-epicatechin-3--gallate in the chromatogram of thecorrespond to those in the chromatogram ofas obtained in the test for

Use an unsaturated chamber, and condition the plate to a relative humidity of about 30% using a suitable device.Develop the chromatograms usinguntil the solvent front has moved about three-fourths of the plate, dry the plate at 100, dip in thedry, and immediately examine the plate under visible lightThe chromatogram is stable up to 30 minutes; afterward, the plate's background darkens significantly.: the chromatogram of theexhibits main bands similar in position and color to the main bands in the chromatogram of the. The chromatogram of theexhibits four main brownish-orange bands withvalues of approximately 0.38, 0.48, 0.52, and 0.62 corresponding to ()-epigallocatechin-3--gallate, ()-epigallocatechin, ()-epicatechin-3--gallate, and ()-epicatechin, respectively. The most intense band is the one for ()-epigallocatechin-3--gallate. The least intense band is the one for ()-epicatechin.

Dissolve 140 mg of fast blue B salt in 10 mL of water, add 140 mL of methanol and 50 mL of dichloromethane, and mix.Prepare weekly and store at 4in the dark.

Test solution— Proceed as directed for Standard solution, except to use Powdered Extract.

Dissolve about 40 mg of USP Powdered Decaffeinated Green Tea Extract RS in 10 mL of a mixture of alcohol and water (8:2) by sonication for 10 minutes, and centrifuge. Use the clear supernatant.Prepare fresh. Store below20, if storage is needed.

The total aerobic microbial count does not exceed 10cfu per g. The total combined yeasts and molds count does not exceed 10cfu per g.

It meets the requirements of the tests for absence ofspecies and

Limit of gallic acid—

Solution A, Solution B, Mobile phase,

and

Chromatographic system —Proceed as directed in the test for

Content of polyphenols

.

Standard solution—

Dissolve an accurately weighed quantity of gallic acid in

Solution A

to obtain a solution having a known concentration of about 0.2 mg per mL.

Test solution—

Transfer about 500 mg of Powdered Extract, accurately weighed, to a 25-mL volumetric flask, dissolve, dilute with

Solution A

to volume, mix, and centrifuge. Additional reading:
White Champaca Leaf (Michelia alba) Wildcrafted ...

Goto chenlv to know more.

Procedure—

Separately inject equal volumes (about 15 µL) of the

Standard solution

and the

Test solution

into the chromatograph, record the chromatograms, and measure the areas of the gallic acid peaks. Separately calculate the percentages of gallic acid in the portion of Powdered Extract taken using the formula:

2500(

C

/

W

)(

r

U

/

r

S

) in which

C

is the concentration, in mg per mL, of gallic acid in the

Standard solution; W

is the weight, in mg, of Powdered Extract taken to prepare the

Test solution; and

r

U

and

r

S

are the peak responses obtained for gallic acid in the

Test solution

and the

Standard solution,

respectively: not more than 1.0% is found.

Separately inject equal volumes (about 15 µL) of theand theinto the chromatograph, record the chromatograms, and measure the areas of the gallic acid peaks. Separately calculate the percentages of gallic acid in the portion of Powdered Extract taken using the formula:in whichis the concentration, in mg per mL, of gallic acid in theis the weight, in mg, of Powdered Extract taken to prepare theandare the peak responses obtained for gallic acid in theand therespectively: not more than 1.0% is found.

Contact us to discuss your requirements of Green Tea Extract Powder. Our experienced sales team can help you identify the options that best suit your needs.

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